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Corning Life Sciences 25-cm 2 cell culture flasks
25 Cm 2 Cell Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/25-cm+2+cell+culture+flask/96+well+plates/pmc11445277-38-6-9
Average 90 stars, based on 1 article reviews
25-cm 2 cell culture flasks - by Bioz Stars, 2026-10
90/100 stars

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Cell Culture:

Article Title: Particulate matter induces cytokine expression in human bronchial epithelial cells.
Article Snippet: The present study was designed to determine cytokines produced by primary human bronchial epithelial cells (HBECs) exposed to ambient air pollution particles (EHC-93).. Cytokine messenger RNA (mRNA) was measured using a ribonuclease protection assay and cytokine protein production by enzymelinked immunosorbent assay.. Primary HBECs were freshly isolated from operated lung, cultured to confluence, and exposed to 10 to 500 g/ml of a suspension of ambient particulate matter with a diameter of less than 10 m (PM 10 ) for 2, 8, and 24 h. The mRNA levels of leukemia inhibitory factor (LIF), granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-1 , and IL-8 were increased after exposure to PM 10 , and this increase was dose-dependent between 100 ( P 0.05) and 500 ( P 0.05) g/ml of PM 10 exposure.

Article Title: REDUCED HAMSTER USAGE AND STRESS IN PROPAGATING LEISHMANIA CHAGASI PROMASTIGOTES USING CRYOPRESERVATION AND SAPHENOUS VEIN INOCULATION
Article Snippet: Aliquots (1 ml) within 2.0-ml cryogenic vials were placed into room temperature Cryo 1C Freezing containers (Nalgene, Rochester, New York), stored 12–24 hr at −80 C, and then stored in the vapor phase of liquid nitrogen. .. Stored cells within vials were recovered by thawing (immersion in 26 C water until fully thawed, approximately 1 min) and then diluted in a 25-cm 2 cell culture flask (Corning Life Sciences, Lowell, Massachusetts) containing 2 ml of HOMEM. ..

Article Title: Characteristics of Stem Cells Derived from the Degenerated Human Intervertebral Disc Cartilage Endplate
Article Snippet: The cells were rinsed twice with phosphate buffered saline (PBS) and resuspended in Dulbecco's modified Eagle's medium/F12 (DMEM/F12, HyClone, USA) supplemented with 10% fetal calf serum (FCS, HyClone, USA) and 100 U/ml penicillin-streptomycin (HyClone, USA). .. All of the nucleated cells were cultured in a 25-cm 2 cell culture flask (Costar Corning, USA) in a humidified atmosphere containing 5% CO 2 at 37°C. ..

Article Title: Long-term establishment, characterization and manipulation of cell lines from mouse basal cell carcinoma tumors.
Article Snippet: .. The cell suspensions were then dispersed into a 25-cm 2 cell culture flask (Corning; Fisher Scientific) for incubation in a 37 C humidified incubator containing 7% CO2. ..

Article Title: Magnetic Enrichment of Dendritic Cell Vaccine in Lymph Node with Fluorescent-Magnetic Nanoparticles Enhanced Cancer Immunotherapy
Article Snippet: .. Next, 1 × 10 6 cells were added to a 25-cm 2 cell culture flask (Corning, NY, USA) with 10 mL of complete medium, and a magnet (N35, 49 mm × 9.3 mm × 4.7 mm) was attached to one side of the flask. .. After 24 h, the magnet was removed, and both sides of the flask were imaged with an upright confocal microscope (NIKON Ni-E, Nikon, Japan).

Article Title: Treatment with suboptimal doses of raltegravir leads to aberrant HIV-1 integrations
Article Snippet: .. PBMCs from healthy human donors were seeded at a minimum of 5 × 10 6 cells per 25-cm 2 cell culture flask (Corning) and maintained in RPMI media supplemented with 10% FBS, 100 U/mL penicillin G, 100 μg/mL streptomycin, 2 mM L-glutamine, and 50 U IL-2. ..

Article Title: A Fluorogenic Far Red-Emitting Molecular Viscometer for Ascertaining Lysosomal Stress in Live Cells and Caenorhabditis elegans
Article Snippet: All the confocal microscopy imaging was performed with an Olympus FV3000 confocal laser scanning microscope. .. BHK-21 and U-87 MG cells were obtained from the National Centre for Cell Science, Pune, India, and were grown in a 25-cm 2 cell culture flask (Corning, United States) using DMEM (phenol red-free) containing 10% (v/v) FBS and 1% (v/v) antibiotic cocktail in 5% CO 2 at 37°C in a CO 2 incubator. ..

Incubation:

Article Title: Long-term establishment, characterization and manipulation of cell lines from mouse basal cell carcinoma tumors.
Article Snippet: .. The cell suspensions were then dispersed into a 25-cm 2 cell culture flask (Corning; Fisher Scientific) for incubation in a 37 C humidified incubator containing 7% CO2. ..



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